The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products and solutions were being digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was introduced by the https://estramustinephosphatesodi10986.timeblog.net/62269641/detailed-notes-on-sr-4835